wash buffer (Thermo Fisher)

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Thermo Fisher wash buffer
Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/washing+buffer/Triton+X-100/pmc13084690-106-8-38
Average 99 stars, based on 1 article reviews

wash buffer - by Bioz Stars, 2026-11

99/100 stars

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Article Title: A rice ceRNA module suppresses Rhizoctonia solani-induced cross-kingdom RNAi to reduce fungal pathogenicity.
Article Snippet: Beads were washed five times (3 min/wash) with washing buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 0.5% Triton X-100), and OsAGOs-bound RNA was extracted using TRIzol (Invitrogen, California, USA).

Article Title: Post-weaning gut microbiota colonization in germ-free mice results in crinophagy-like granule abnormalities and apoptosis in Paneth cells.
Article Snippet: After washing with washing buffer (0.2% Triton X-100 and 3% NaCl in PBS) overnight at 4 ◦C, secondary antibody reaction was performed with Alexa Fluor 594 goat anti-rabbit IgG (1:400; Thermo Fisher Scientific) diluted in antibody dilution buffer overnight at 4 ◦C.

Enzyme-linked Immunospot:

Article Title: High-affinity TCR for recognizing AFP antigen
Article Snippet: .. Reagents Assay medium: 10% FBS (Gibco, Cat No. 16000-044), RPMI 1640 (Gibco, Cat No. C11875500bt) Washing buffer (PBST): 0.01M PBS/0.05% Tween 20 PBS (Gibco, Cat No. C10010500BT) PVDF ELISPOT 96 well-plate (Merck Millipore., Cat No., MSIPS4510) Human IFN-γ ELISPOT PVDF-Enzyme Kit (BD) contains all the other necessary reagents (capture and detection antibodies, streptavidin-alkaline phosphatase and BCIP/NBT solution) Method Target Cells The target cells used in this experiment were T2 cells loaded with specific short peptides of FMNKFIYEI (SEQ ID NO: 25). ..

Membrane:

Article Title: Formulations comprising PCSK9 specific monoclonal antibodies
Article Snippet: 100 μg of protein was denatured at 70° C. for 10 minutes and separated on 4-12% Bis-Tris SDS gradient gel (Invitrogen). .. Proteins were transferred to a 0.45 μm PVDF membrane (Invitrogen) and blocked in washing buffer (50 mM Tris PH7.5, 150 mM NaCL, 2 mM CaCl₂and 0.05% Tween 20) containing 5% non-fat milk for 1 hour at room temperature. .. The blot was then probed with goat anti-mouse LDLR antibody (R&D system) 1:2000 or anti-β actin (sigma) 1:2000 for 1 hour at room temperature.

Incubation:

Article Title: Tuft cells shape airway remodeling by eliciting OXGR1- and SOX9-dependent stem cell programs.
Article Snippet: The tracheas were incubated overnight at 4 °C for 48 h with the specified primary antibodies: anti-DCLK1 (1:100, Rabbit, Abcam), and anti-GNAT3 (1:100, Goat, MyBioscience). .. The tracheas were rinsed with washing buffer (PBS containing 0.2% Triton X-100 and 0.1% Tween 20) for 1-2 h at 4 °C, followed by incubation with the appropriate combinations of secondary antibodies along with DAPI (1:500, Sigma-Aldrich): donkey anti-rabbit AF488 (1:200, Invitrogen), and donkey anti-goat AF594 (1:200, Invitrogen). .. Finally, the tracheas were longitudinally split into two halves, with the epithelial surface facing upward, and embedded using VECTASHIELD Vibrance Antifade Mounting medium with DAPI (Vector).

Blocking Assay:

Article Title: Targeted degradation of Drosophila FOG homolog U-shaped mimics macrophage transdifferentiation in S2 cells.
Article Snippet: .. After washing the membranes four times for 5 min at room temperature in washing buffer (PBS, 0.1% (w/v) Tween-20) appropriate HRP-coupled secondary antibodies (anti-mouse IgG (NA931, GE Healthcare), anti-rabbit IgG (NA934, GE Healthcare) and anti-rat IgG (31470, Thermo Fisher Scientific) were applied in blocking buffer for 2 h at room temperature. .. After four washing cycles for 5 min in washing buffer Western blot signals were detected by chemiluminescence using the Immobilon Western Blot Chemiluminescence HRP substrate (WBKLS0500, Millipore).

Lysis:

Article Title: Oncogenic E3-ligase adaptors MAGE-A3/6 promote cancer cell migration via BAP18 degradation
Article Snippet: .. Beads were washed three times using IP lysis buffer as washing buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 1 mM EDTA, Thermo Scientific PierceTM, #87788). .. Bound protein was eluted by boiling for 10 minutes at 95°C using 2x XT sample buffer (Bio-Rad, #1610791), containing 100 mM DTT (Bio-Rad, #1610611).

Control:

Article Title: Unraveling the dual immunomodulatory and immunogenic roles of the central conserved cysteine-rich region in respiratory syncytial virus G protein
Article Snippet: .. To ensure assay specificity, it was initially validated by comparing serum reactivity against the RSV G protein and an unrelated control protein (MBP), confirming specific and selective recognition of RSV G. Plates were washed three times with washing buffer (TBS + 0.1% Tween-20) and blocked for 1 h at room temperature (RT) with 1% BSA in TBS (Gibco). ..

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